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Point mutations in ROGFP2 WT -PFGRX C32S/C88S , ROGFP2 C151S or ROGFP2 C208S were introduced by PCR with Pfu polymerase using ROGFP2 WT -PFGRX C32S /pET45b or ROGFP2 /pET28a as a template

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Plant GSTs Plant GSTs are a family of multifunctional enzymes involved in the intracellular detoxification of xenobiotics and toxic compounds produced endogenously [54], [26]

These observations suggested that TRP14 could be the rate-limiting enzyme for intracellular cystine reduction and that this activity could not be fulfilled by Trx1 alone within the cellular context